Our in vitro metabolism services provide deep insights into the metabolic fate of your compound across liver and extrahepatic tissues.
Using hepatocytes, microsomes, recombinant enzymes, and advanced UPLC-HR/MS technology, we deliver high-quality data on clearance, metabolite formation, enzyme phenotyping, and reactivity. These studies support risk assessment, DDI evaluation, and regulatory submissions, helping you de-risk development and accelerate decision-making.
Service List
Metabolic Stability
Assess compound stability using hepatocytes, microsomes, S9 fractions, or extrahepatic tissues. We provide in vitro clearance, half-life determination, and IVIVE modelling to predict hepatic clearance and bioavailability.
Metabolite Identification and Profiling
Identify and characterize metabolites across species and tissues with UPLC-HR/MS. We pinpoint metabolic soft-spots, compare profiles for toxicity studies, and isolate metabolites for further evaluation.
Metabolite Synthesis
Produce identified metabolites via chemical synthesis or microbial biotransformation when direct synthesis is not feasible. Available for qualitative and quantitative applications.
Identification of Metabolising Enzymes
Use recombinant enzymes, microsomes, and selective inhibitors to phenotype CYP and non-CYP enzymes. Supports DDI risk assessment and evaluation of interindividual clearance variability.
Non-CYP Mediated Metabolism
Investigate conjugative and oxidative pathways mediated by UGT, SULT, NAT, FMO, MAO, CE, AOX, XO, and others. Essential for compounds where CYP metabolism is not dominant.
Extrahepatic Metabolism
Evaluate metabolism in intestine, kidney, skin, and plasma. These studies complement hepatic assays to reveal tissue-specific contributions to clearance.
Metabolite Production and NMR Identification
Upscale metabolite production for structure confirmation, quantification, and use as reference materials. NMR and MS provide precise structural characterization.
Reactive Metabolite Screening
Detect and characterize reactive intermediates using GSH and other trapping agents, supported by high-resolution LC-MS. Optionally, assess covalent binding with radiolabeled compounds.
Acyl-Glucuronide Reactivity
Evaluate the reactivity and stability of acyl-glucuronides without requiring synthesized standards. Measure acyl migration and formation of reactive aldehyde isomers to assess potential toxicity risk.
